Modified RNA guides improve gene targeting for next-generation CRISPR tools and therapies.
In the latest of ongoing efforts to expand technologies for modifying genes and their expression, researchers in the lab of Neville Sanjana, PhD, at the New York Genome Center (NYGC) and New York University (NYU) have developed chemically modified guide RNAs for a CRISPR system that targets RNA instead of DNA. These chemically-modified guide RNAs significantly enhance the ability to target – trace, edit, and/or knockdown – RNA in human cells.
Drawing on the lab’s previous study that outlined principles for optimal Cas13 guide design, published in Nature Biotechnology in March 2020, the researchers systematically applied and tested a variety of chemical modifications.
For example, they found that adding 3 bases with a different type of chemical bond linking them to each other (phosphorothioate modification) extended RNA target knockdown ability by several days in a human cell line. In primary T cells, the phosphorothioate modification resulted in 60-65% knockdown of expression of CD46, a receptor involved in immune system regulation, as compared with achieving 40-45% knockdown when using an unmodified guide .
The team also found that certain methylation and inverted terminator modifications also improved Cas13 activity. For all modifications, the placement of these modified RNA bases was also crucial. When placed incorrectly, the modifications resulted in guide RNAs that did not function.
“These modified guides further expand the toolbox for genome and transcriptome engineering…”
“We hope the improved effectiveness and stability from these modified CRISPR Cas13 guides will help pave the way for use of RNA-targeting CRISPRs in primary cells,” says Hans-Hermann Wessels, PhD, a postdoctoral scientist in the lab who is a co-first author of the study .
“These modified guides further expand the toolbox for genome and transcriptome engineering. For non-coding elements in the human genome, targeting DNA may not be effective, and other organisms, such as RNA viruses like coronavirus or flu, cannot be targeted at all,” said Dr Sanjana, Core Faculty Member, NYGC, Assistant Professor of Biology, NYU, and Assistant Professor of Neuroscience and Physiology, NYU Grossman School of Medicine, the study’s senior author .
The team’s test to knockdown the universal leader sequence segment of the RNA virus SARS-CoV-2 in human cells, for example, is only possible using an RNA-targeting CRISPR like Cas13. SARS-CoV-2 enters the cells and releases its RNA genome, which is then transcribed into smaller RNAs, referred to as subgenomic RNAs. These subgenomic RNAs are responsible for making the different proteins required for the virus to replicate and then infect other cells. The universal leader sequence is found at the beginning of each subgenomic RNA. Thus, an effective approach for targeting the universal leader sequence may protect cells against further viral replication and infection.
Also of key import is CRISPR-Cas13’s ability to modulate genetic expression without permanently altering the underlying DNA genome sequence, as do DNA-targeting CRISPRs like Cas9 or Cas12a. “Transient modulation to spur genetic expression outcomes is often preferred in biomedical research and drug development. For example, the messenger RNA vaccines for SARS-CoV-2 express transiently but create an immune memory that lasts beyond their expression lifetime,” notes Dr Sanjana .