Discovery of mitochondrial waste disposal mechanism could open up new therapeutic options

Research team identifies how cells’ powerhouses get rid of mutated mtDNA, identifying a new molecular target that could lead to treatment for age-associated diseases.

Scientists at the University of Cologne have discovered how cells can eliminate mutated mitochondrial DNA (mtDNA). Mitochondria are the powerhouses of our cells, and due to their evolutionary descent from bacteria, they still have genetic material packaged in chromosome-like structures (nucleoids).

Longevity.Technology: Mitochondria convert oxygen and the chemical energy in our food into a biologically usable form. Now a team of researchers from the University of Cologne’s Physiology Centre at the Faculty of Medicine, the Centre for Molecular Medicine Cologne (CMMC) and the CECAD Cluster of Excellence for Aging Research has shown that mutations of the mtDNA lead to a local rearrangement of proteins in the mitochondrial membrane. The mutated mtDNA is targeted, eliminated and subjected to autophagy, the cellular ‘waste disposal’. The results have been published in Nature Communications.

In many tissues, mutations in mtDNA accumulate as a result of normal aging, and these kinds of mutations are an important cause of many aging-associated diseases. There are thousands of copies mtDNA in every cell, so mitochondrial function is only impaired when the percentage of mutated mtDNA molecules exceeds a certain threshold value. It has long been established that mitochondrial damage, including acute mtDNA damage, triggers mitophagy, a process whereby dysfunctional mitochondrial parts are selectively degraded and recycled.

“What is new in our study is that this mechanism does not affect the cells’ endowment with mitochondria, but only clears out the damaged mtDNA,” explained Dr David Pla-Martin, lead author on the study.

“By labelling neighbouring proteins – so-called proximity labelling – we showed that mtDNA damage leads to the recruitment of endosomes in close proximity to nucleoids [1].”

Their removal is coordinated by the interaction of the nucleoid protein Twinkle (excellent choice of name!) and the mitochondrial membrane proteins SAMM50 and ATAD3. ATAD3 controls their distribution, SAMM50 induces the release and transfer of the nucleoid to the so-called endosomes.

“This additionally prevents the activation of an immune response. The protein VPS35, the main component of the retromer, mediates the maturation of early endosomes into late autophagy vesicles, where degradation and recycling ultimately take place,” said Pla-Martin [1].

Using a mouse model in which mtDNA mutations lead to impaired muscle regeneration, the scientists were able to demonstrate that selectively mutated mtDNA can be removed by stimulating the activity of the autophagy mechanism with rapamycin. The total number of mtDNA copies thus remains constant, preserving mitochondrial function [2].

Group leader Professor Dr Rudolf Wiesner added: “Mutations in the genes that code for these proteins lead to severe neurological diseases, in VPS35 for example Parkinson’s disease. We now want to use these proteins as new molecular targets to open up entirely new treatment options for these kinds of aging-associated diseases [1].”

Although the road to therapeutic application could still be long, the research team sees a promising approach in this work.